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[1]黄金娟,隋 御,李元杰,等.REV3基因联合抗癌药物对SW-480细胞的影响[J].宁夏医科大学学报,2012,(06):561-564.
 HUANG Jin-juan,SUI Yu,LI Yuan-jie,et al.Influence of REV3 Gene Combined with Anticancer Drugs on Proliferation and Apoptosis of the SW-480 Cells[J].Ningxia Medical University,2012,(06):561-564.
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REV3基因联合抗癌药物对SW-480细胞的影响(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2012年06期
页码:
561-564
栏目:
论著
出版日期:
2012-06-30

文章信息/Info

Title:
Influence of REV3 Gene Combined with Anticancer Drugs on Proliferation and Apoptosis of the SW-480 Cells
作者:
黄金娟 隋 御 李元杰 金彩霞 张竞文 贾 伟 李利坚 周 翔 徐 方
宁夏医科大学基础医学院医学遗传学与细胞生物学系、生育力保持省部共建教育部重点实验室,银川 750004
Author(s):
HUANG Jin-juan SUI Yu LI Yuan-jie JIN Cai-xia ZHANG Jing-wen JIA Wei LI Li-jian ZHOU Xiang XU Fang
Medical Genetics and Cell Biology Department, Ningxia Medical University, Yinchuan 750004
关键词:
REV3基因 RNAi技术 SW-480 qRT-PCR 流式细胞仪
Keywords:
REV3 gene RNA interference colon carcinoma cell(SW-480) real-time RT-PCR flow cytometry
分类号:
R979.1
DOI:
-
文献标志码:
A
摘要:
目的 探讨REV3基因低表达联合抗癌药物对人结肠癌细胞(SW-480)增殖和凋亡的影响。 方法 利用RNAi技术降低REV3基因在SW-480细胞中的表达,以实时荧光定量PCR和免疫细胞化学检测REV3表达量的降低情况,选择低表达效率具有统计学意义的细胞作为实验组细胞,再用抗癌药物作用48h后运用MTT实验和流式细胞仪,对实验组和对照组细胞进行细胞增殖和凋亡变化情况的检测。 结果 与空白对照组相比,REV3基因低表达或抗癌药物(顺铂或槐定碱)单独作用均能促进SW-480细胞凋亡并抑制其增殖(P<0.01); 实验组内,与药物空白组相比,REV3基因低表达的同时再联合一种抗癌药物(顺铂或槐定碱),对SW-480细胞凋亡和增殖的作用较单一因素更加显著; 若将三者联合则比以上任一两种联合作用效果更为显著,以上结果均具有统计学意义(P<0.05)。结论 REV3 基因低表达、顺铂、槐定碱或这三因素两两联合均可促进结肠癌SW-480细胞的凋亡并抑制其增殖,若三者同时联合作用效果更加显著,提示“基因+药物”联合抑癌具有协同增效的作用。
Abstract:
Objective To explore influence of REV3 gene combined with anticancer drugs on proliferation and apoptosis of colon cancer cells. Methods The REV3 expression in SW-480 cells were experimentally suppressed by the interference RNA technology(RNAi)and were monitored by real-time RT-PCR and immunocytochemistry. Compared with untreated or mock-treated cells, cells with significant reduction of REV3 expression were taken as treatrment groups. Anticancer drugs were used to treat control and REV3-depleting cells and the single and combined effects were measured by flow cytometry and MTT 48 h after treatments. Results Compared with qRT-PCR control groups, ablation of REV3 or treatment of anticancer drugs(DDP or Sophoridine)alone promoted significantly SW-480 cell apoptosis and inhibit cell proliferation(P<0.01). REV3 depletion in combination with treatment of either drug significantly enhanced the biological effects(P<0.05). Furthermore, the combined effects of REV3 depletion and treatment with both drugs were still additive. Conclusion Ablation of REV3 significantly enhances treatments of anticancer drugs DDP and qRT-PCR Sophoridine on colon cells regardless of the drugs being applied individually or jointly. This finding suggests synergistic interactions between anticancer drugs and genetic variations, and offers a guideline for cancer chemotherapy.

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备注/Memo

备注/Memo:
收稿日期:2011-12-20 基金项目:国家自然科学基金项目(81060170); 教育部“春晖计划”项目(Z2011056,Z2008-1-75015); 宁夏医科大学校级科研项目(XM200704) 作者简介:黄金娟, 女, 在读硕士研究生。E-mail:njj55@163.com 通信作者:徐方, 女, 教授, 硕士研究生导师。E-mail: xufang@nxmu.edu.cn
更新日期/Last Update: 2012-06-20