|本期目录/Table of Contents|

[1]秦三利,杨志伟.真核重组质粒pEGFP-FcγRIIb在NIH3T3细胞及 BALB/c小鼠体内的表达[J].宁夏医科大学学报,2012,(01):4-6.
 QIN San-li,YANG Zhi-wei.Expression of Eukaryotic Recombinant Plasmid pEGFP-FcγRIIb in Eukaryotic Cells and BALB/c Mice[J].Ningxia Medical University,2012,(01):4-6.
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真核重组质粒pEGFP-FcγRIIb在NIH3T3细胞及 BALB/c小鼠体内的表达(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2012年01期
页码:
4-6
栏目:
论著
出版日期:
2012-01-30

文章信息/Info

Title:
Expression of Eukaryotic Recombinant Plasmid pEGFP-FcγRIIb in Eukaryotic Cells and BALB/c Mice
作者:
秦三利 杨志伟
宁夏医科大学基础医学院病原生物学与免疫学系,银川 750004
Author(s):
QIN San-li YANG Zhi-wei
Dept.of Pathogenic Biology and Immunology, Ningxia Med.Univ., Yinchuan 750004
关键词:
FcγRIIB pEGFP-FcγRIIb BALB/c小鼠
Keywords:
FcγRIIB pEGFP-FcγRIIb BALB/c mice
分类号:
RQ754
DOI:
-
文献标志码:
A
摘要:
目的 观察真核重组质粒pEGFP-FcγRIIb在真核细胞NIH3T3及BALB/c小鼠体内的表达,为后续的体内研究奠定基础。 方法 采用脂质体法将重组质粒pEGFP-FcγRIIb转染到NIH3T3中,G418筛选3周后,采用Western blot方法鉴定稳定表达的产物。进一步将重组质粒注射入BALB/c小鼠肌肉中,用Western blot及免疫组织化学法检测人FcγRIIB的表达情况。 结果 转染重组质粒pEGFP-FcγRIIb的NIH3T3细胞及注射重组质粒的小鼠肌肉中表达了人的FcγRIIB蛋白,分子量约为37kDa。 结论 成功获得了真核重组质粒pEGFP-FcγRIIb在NIH3T3及BALB/c小鼠体内的表达,为进一步研究FcγRIIB的功能奠定了基础。
Abstract:
Objective To detect the gene expression of eukaryotic recombinant plasmid pEGFP-FcγRIIb in eukaryotic cells and BALB/c mice, and to establish a foundation for the further study in vivo. Methods The NIH3T3 cells were transfected by the recombinant plasmid of pEGFP-FcγRIIb with lipofection strategy, using G418 to select positive cells for three weeks. The total protein was analyzed by Western blot to detect stable products. The BALB/c mice were injected intramuscularly with the pEGFP-FcγRIIb, then the expression of human FcγRIIB was detected with Western blot and Immunohistochemistry. Results About 37 kDa protein of human FcγRIIB was detected in stable transfected NIH3T3 cells and in BALB/c mice which were injected the pEGFP-FcγRIIb. Conclusion The success in expression of the eukaryotic recombinant plasmid pEGFP-FcγRIIb in the NIH3T3 cells and BALB/c mice make it possible to study further.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2011-06-20 基金项目:国家自然基金(81160375); 宁夏医科大学博士启动金(XT200905) 作者简介:秦三利(1986-),女,内蒙古人,硕士,从事感染与免疫研究。 通信作者:杨志伟,教授,硕士研究生导师。 E-mail:yangzhw0817@163.com
更新日期/Last Update: 2012-01-20