|本期目录/Table of Contents|

[1]丁淑琴,史彦奎,杨圆圆,等.生物信息学技术在结核分支杆菌ESAT-6 重组抗原研究中的应用[J].宁夏医科大学学报,2010,(03):334-336.
 DING Shu-qin,SHI Yan-kui,YANG Yuan-yuan,et al.Application of Bioinformatics in the Study ofMycobacterium Tuberculosis Recombinant Antigen ESAT-6[J].Ningxia Medical University,2010,(03):334-336.
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2010年03期
页码:
334-336
栏目:
论著
出版日期:
2011-12-30

文章信息/Info

Title:
Application of Bioinformatics in the Study ofMycobacterium Tuberculosis Recombinant Antigen ESAT-6
作者:
丁淑琴1 史彦奎1 杨圆圆2 杨凤琴1
1.宁夏医科大学,银川 750004; 2.银川市第一人民医院,银川 750001
Author(s):
DING Shu-qin1 SHI Yan-kui1 YANG Yuan-yuan2 YANG Feng-qin1
1.Ningxia Medical University, Yinchuan 750004; 2.Yinchuan First People's Hospital,Yinchuan 750001
关键词:
生物信息学 结核分支杆菌 ESAT-6 重组抗原
Keywords:
bioinformatics mycobacterium tuberculosis ESAT-6 recombinant protein
分类号:
R378.91+1
DOI:
-
文献标志码:
A
摘要:
目的 应用生物信息学分析软件预测分析结核分支杆菌ESAT-6基因及相关蛋白的特性。方法 应用NCBI、Expasy等在线生物信息学网站及DNAstar、Rasmol等软件包分析ESAT-6并进行同源比对; 预测二级、三级结构,以及预测主要抗原表位等。结果 结核分支杆菌重组抗原ESAT-6与已发表氨基酸序列同源性为90%。预测该蛋白分子质量约为9885.7Da,PI为4.6,4个抗原表位,其结构域位于56-87位。结论 生物信息学技术在结核分支杆菌ESAT-6重组抗原研究中有一定的理论和应用价值。
Abstract:
Objective To predict the structure and function of recombinant antigen ESAT-6 of Mycobacterium tuberculosis using bioinformatics method. Methods By online analysis at bioinformatics websites such as NCBI(http://www.nibi.nlm.nih.gov/)and Expasy(http://cn.expasy. org/), and employing software packages such as DNAstar and Rasmol to do multi-sequence homological alignment, secondary structure and tertiary structure,antigenic epitope analysis,etc. Results Compared with the amino acid sequence of ESAT-6 of Mycobacterium tuberculosis published, the homologies was 90%. Analysis of the predicted protein indicated a molecular mass of 9885.7 KDa, PI was 4.6, function sites and four antigen pitope were found. Conclusion Bioinformatic is valuable to the study ESAT-6 of Mycobacterium tuberculosis.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2009-10-23 基金项目:宁夏医科大学校级项目基金资助(项目编号:XM200825)。 作者简介:丁淑琴(1974-),女(回族),宁夏人,讲师,硕士。 E-mail:md-dshn@163.com
更新日期/Last Update: 2010-03-20