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[1]才春东,姜 宁,王国增,等.枸橼酸钾对大鼠肾草酸钙结石骨桥蛋白表达的影响[J].宁夏医科大学学报,2010,(02):176-178.
 CAI Chun-dong,JIANG Ning,WANG Guo-zeng,et al.Effects of Citrate on Osteopontin Expression of UrinaryOxalate Calcium StoneFormation in Rats[J].Ningxia Medical University,2010,(02):176-178.
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枸橼酸钾对大鼠肾草酸钙结石骨桥蛋白表达的影响(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2010年02期
页码:
176-178
栏目:
论著
出版日期:
2011-12-30

文章信息/Info

Title:
Effects of Citrate on Osteopontin Expression of Urinary Oxalate Calcium StoneFormation in Rats
作者:
才春东1 姜 宁12 王国增2 郑景存2
1.宁夏医科大学,银川 750004; 2.上海市浦东新区公利医院泌尿外科,上海 200135
Author(s):
CAI Chun-dong1JIANG Ning12WANG Guo-zeng2ZHENG Jing-cun2
1.Ningxia Med.Univ.,Yinchuan 750004; 2.Gongli Hospital of Shanghai,Shanghai 200135
关键词:
枸橼酸钾 骨桥蛋白 肾结石
Keywords:
citrate OPN CaOx stone
分类号:
R691.4
DOI:
-
文献标志码:
A
摘要:
目的 探讨枸橼酸钾对大鼠肾草酸钙结石骨桥蛋白(osteopontn,OPN)表达的影响。方法 32只8周龄雄性Wistar大鼠随机分为正常对照组、枸橼酸钾组、结石组(乙二醇和氯化氨)、结石+枸橼酸钾组,每组8只,采用乙二醇和氯化铵诱导大鼠肾草酸钙结石模型进行试验研究。连续给药4周后处死大鼠取肾组织,采用HE染色和免疫组化法检测各组肾结石形成及OPN表达情况。结果 结石+枸橼酸钾组大鼠肾结石形成比结石组明显减少(P<0.05); 结石+枸橼酸钾组大鼠的OPN表达比结石组明显降低(P<0.05); 枸橼酸钾组OPN表达与对照组相比无明显变化( P>0.05)。结论 枸橼酸钾可能通过减少草酸钙结晶的形成而减轻对肾小管上皮细胞的损伤,降低肾OPN的表达。
Abstract:
Objective To examine the effect of citrate on osteopontin(OPN)expression of urinary oxala te calcium(CaOx)stone formation in rats.Methods thirty-two a dult male wistar strain rats were randomly divided into four groups(n=8 each): normal control group、the citrate group、stone formation group(ethylene glycol(EG)and ammonium chloride)and the stone formation-citrate group.After 4 weeks medication,the rats were killed and kedney was used for histological examination.The formation of renal stone and the expression of OPN in rat renal tissue of each group were determined with HE staining and Immunohistochemistry methods.Results The formation of renal stone in the stone formation-citrate group wassignificantly lower than that in the stone group.The expression of OPN in thestone formation-citrate group was significantly less than that in the stone group.However those in the contol group and in cirate group showed the same.Conclusion Citrate can reduce crystal formation and crystal damaging on renal duct and decrease OPN expression.

参考文献/References:

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[9] 米其武,曹正国,刘继红,等. 泽泻有效部位对肾草酸钙结石模型大鼠肾组织骨桥蛋白表达的影响[J]. 中草药,2005,36(12): 1827-1831.
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备注/Memo

备注/Memo:
收稿日期:2009-07-04 作者简介:才春东(1979-),男,河北人,在读硕士研究生,主要从事泌尿系结石的预防与治疗方面的研究研究工作。 通信作者:姜宁,男,主任医师,硕士研究生导师,主要从事泌尿系结石的预防与 治疗方面的研究工作。
更新日期/Last Update: 2010-02-20