|本期目录/Table of Contents|

[1]马彦,王振海,孔繁元,等.宁夏地区黄牛博尔纳病病毒自然感染状况的探讨[J].宁夏医科大学学报,2010,(01):22-25.
 MA Yan,WANG Zhen hai,KONG Fan yuan.BDV Natural Infection in Blood of Cows in Ningxia[J].Ningxia Medical University,2010,(01):22-25.
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宁夏地区黄牛博尔纳病病毒自然感染状况的探讨(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2010年01期
页码:
22-25
栏目:
论著
出版日期:
2011-12-30

文章信息/Info

Title:
BDV Natural Infection in Blood of Cows in Ningxia
作者:
马彦1王振海2孔繁元2谢鹏3
1宁夏医科大学附属石嘴山市第一人民医院神经内科,石嘴山 753200; 2宁夏医科 大学附属医院神经内科, 银川 750004; 3重庆医科大学附属第一医院神经内科,重庆 400016
Author(s):
MA Yan WANG Zhenhai KONG Fanyuan
Department of Neurology, the Affiliated Hospital of Ningxia Medical Un iversity, Yinchuan 750004
关键词:
博尔纳病病毒荧光定量巢式逆转录酶聚合酶链反应牛外 周血宁夏
Keywords:
borna disease virus fluorescence quantitative nested reverse transcriptase PCR PBMC of cowNingxia
分类号:
R938.2
DOI:
-
文献标志码:
A
摘要:
目的 了解宁夏地区黄牛博尔纳病病毒(Borna disease virus,BD V)的自然感染情 况,对检测到的BDV p24基因阳性扩增片段进行核苷酸序列比对,探讨其与国际标准病毒株 及其分离株的同源性。方法 采用荧光定量巢式逆转录酶聚合酶链反应(FQ -nRT-PC R)检测宁夏地区205例健康牛外周血单个核细胞(PBMCs)BDVp24基因片段,并对其中的阳性扩 增产物进行基因序列测定,然后应用BLAST软件以及DNAsist 50 软件对测序结果分析。[H T H]结果 205例中3例阳性,阳性率为15%;该BDVp24 片段的核苷酸序列与Stra in H3915 同源性 最高达9767%,与标准病毒株 H1766同源性为9651%,与Strain V/FR的同源性为9535 % ,且它们编码的氨基酸相同。结论 宁夏地区黄牛中存在动物源性BDV的 自然感染,该BDV p24核苷酸序列与标准病毒株具有高度同源性。
Abstract:
Objective To investigate the natural infection of BDV in cows of Ning xia and to explore if the nucleotide sequence in BDV p24 was homophylic with the overseas strains detected from human and animals. Methods The p24 fragment of BDV was detected by nested reverse transcriptase polymerase chain reaction with Fluorescence Quantitative PCR(FQnRTPCR) in PBMCs from 205 cows. The products o f FQnRTPCR from positive samples were cloned and sequenced before compared w it h those of the overseas strains by BLAST and DNAsist 5.0. Results T here were 3 p ositive(1.5%). BDV p24 in cows from Ningxia was homophylic with strain V/FR( 95. 35%),strain H1766(96.51%) and strain H3915(97.67%). However, their amino acid sequence remained the same. Conclusion There is BDV natural infec tion probably originated from cows of Ningxia. The gene sequence is high homophylic with that of standard strain.

参考文献/References:

[1] Gonzalez-Dunia D,Volmer R,Mayer D,et alBorna disease virus int erference with neuronal plasticity[J].Virus Res,2005,111(2):224-234
[2] Gonzalez-Dunia D,Watanabe M,Syna S,et alSynaptic Pathology in Borna Disease Virus Persistent Infection[J].J Virol,2000,74(8):3441-3448
[3] Salvatore M,Morzunov S,Schwemmle M,et alBorna disease virus in brai ns of North American and European people with schizophrenia and bipolar disorder[J] .Lancet,1997,349(9068):1813-1814
[4] Rott R,Becht HNatural and experimental Borna disease in animals[J].C urr Top Microbiol Immunol,1995,190:17-30
[5] Hagiwara K,Asakawa M,Liao LSeroprevalence of Borna disease virus in d omestic animals in Xinjiang,China[J].Vet Microbiol,2001,80(4):383-389
[6] 马培林,张凤民,李桂梅,等博尔纳病病毒自然感染状况及其核苷酸序列[J]. 中国公共卫生,2004,20(4):408-410
[7] 王振海,谢鹏,韩玉霞,等,宁夏及其周边地区博尔纳病病毒感染的分子流行病学 研究[J].中华流行病学杂志,2006,27(6):479-482
[8] 赵立波,谢鹏,牟君,等重庆市山羊博尔纳病病毒P24基因的检测[J].中国兽医 科学,2006,36(6):460-463
[9] Chalmers RM,Thomas DR,Salmon RLBorna disease virus and the evidence for human pathogenicity:a systematic review[J].QJM,2005,98:255-274〖ZK) 〗
[10] [ZK(#]Thomas DR,Chalmers RM,Crook B,et alBorna disease virus and me ntal health:a cross-sectional study[J].QJM,2005,98:247-254

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备注/Memo

备注/Memo:
收稿日期:2009-07-13 基金项目:2006年国家“863”计划项目(2006AA02Z196),2007年国家自然科学基 金(30760067),2007年宁夏自然科学基金项目(NZ0790)联合资助。 作者简介:马彦(1974-),女,主治医师,硕士,从事中枢神经系统感染的研究工 作。E-mail:mayan7475@yahoo.cn 通信作者:王振海,男,副教授,博士,从事中枢神经系统感染性研究工作。
更新日期/Last Update: 2010-01-20