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[1]王娅娜,赵嘉庆,丁淑琴,等.细粒棘球蚴中国大陆株铁蛋白基因分子克隆和序列分析[J].宁夏医科大学学报,2005,(04):253-255,261.[doi:10.3969/j.issn.1674-6309.2005.04.001]
 WANG Ya na,ZHAO Jia qing,DING Shu qin,et al.Cloning and Sequence Analyzing of The Ferritin Gene From Echinococcus Granulosus of China[J].Ningxia Medical University,2005,(04):253-255,261.[doi:10.3969/j.issn.1674-6309.2005.04.001]
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细粒棘球蚴中国大陆株铁蛋白基因分子克隆和序列分析(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2005年04期
页码:
253-255,261
栏目:
论著
出版日期:
2005-07-20

文章信息/Info

Title:
Cloning and Sequence Analyzing of The Ferritin Gene From  Echinococcus Granulosus of China
作者:
王娅娜1赵嘉庆2丁淑琴1王健1赵巍1
1宁夏医学院中心实验室,银川750004;2宁夏医学院人体寄生虫学教研室,银川750004
Author(s):
WANG Yana1 ZHAO Jiaqing2 DING Shuqin1 WANG Jian1 ZHAO Wei1
1Center Laboratory of Ningxia MedColl Yinchuan 750004;2 Human Parasitology  Department of Ningxia Med Coll, Yinchuan 750004
关键词:
细粒棘球蚴铁蛋白基因克隆序列分析
Keywords:
echinococcus granulosus ferritin gene cloning sequencing
分类号:
R383.3+3
DOI:
10.3969/j.issn.1674-6309.2005.04.001
文献标志码:
A
摘要:
目的获得细粒棘球蚴(Egranulosus)铁蛋白(ferritin)基因序列,进行序列分析。方法从包虫病患者 体内获取细粒棘球蚴原头蚴提取总RNA,根据国外细粒棘球蚴铁蛋白基因的已知序列设计一对引物,采用RT-PCR技术扩增出细粒棘球蚴 中国大陆株铁蛋白基因,待PCR产物纯化后,将其克隆到pGEM-T载体,进行序列测定和分析。结果用RT-PCR成功扩增出细粒棘球蚴中国 大陆株铁蛋白基因,测序表明该基因为562bp。同源性比较结果表明: 该基因序列与已发表基因核苷酸序列相比,390bp同源性约为97 7%,推导编码氨基酸序列同源性为977%,此基因序列在435bp出现终止密码。结论测序的铁蛋白基因序列有完整的编码框, 为 进一步研究其结构和功能,以及对包虫病的免疫预防具有重要意义。
Abstract:
Objective To obtain and analyze the sequence of ferritin gene Methods Total RNA was extracted from protoscolexes of cysts from human origin The specific primers were designed according to published nucleotide sequence in the Genebank databaseThe ferritin gene of Echinococcus granulosus was amplified by RTPCR and cloned into pGEMT vector for sequencing and analyzing Results A cDNA sequence of 562bp has been amplified successfully by RTPCR Comparision 390bp of the DNA and amino acid sequence deduced from cDNA with the published ferritin gene sequence of Echinococcus granulosus in the Genebank revealed identity of 977%,it had a terminate code at 435bp Conclusion The ferritin sequence gene has a completely open reading frame Egranulosus ferritin gene gained from protoscolexes can be used as candidate antigen gene to develop vaccine and further biological functions studying

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2005-03-07 基金项目:国家自然科学基金(30260105) 作者简介:王娅娜(1978-),女,陕西人,在读研究生,从事寄生虫病分子疫苗研究; 赵巍(1960-),男,河南人,教授,硕士,硕士研究生导师,从事分子生物学和寄生虫病分子疫苗研究。
更新日期/Last Update: 2005-07-20